Validating internal controls for quantitative plant gene expression studies jordane crantelle dating
The new platform complements existing microarray-based expression profiling techniques, by allowing the analysis of lowly expressed transcription factor genes to determine their involvement in developmental or physiological processes.
We expect that this resource will be of broad utility to the scientific community in the further development of rice as an important model for plant science.
Rice (Oryza sativa L.) is an important crop and the most advanced model for monocotyledonous species; its nuclear genome has been sequenced and molecular tools are being developed for functional analyses.
However, high-throughput methods for rice research are still limited and a large-scale q RT-PCR platform for gene expression analyses has not been reported.
Results A total of 35 Aux/IAA and 39 ARF genes were identified in the Populus genome...
Background Real-time reverse transcription PCR (RT-PCR) has greatly improved the ease and sensitivity of quantitative gene expression studies.
Based on extensive experience with transgenic poplars in laboratory and field environments, we have found that transformation is an extremely useful tool for research in biotechnology and functional genomics.
The key lessons from our experience are: (1) stable gene expression is the rule in vegetatively propagated transgenic poplars; (2) somaclonal variation is modest and...
The terminal region of the developing shoot provides a steep developmental...We demonstrate this resource allows specific and reliable detection of the expression of transcription factor genes in rice.Multi-parallel q RT-PCR allows the versatile and sensitive transcriptome profiling of large numbers of rice transcription factor genes.However the usefulness of these is often limited by their sensitivity and accuracy, particularly for low-abundance transcripts.In contrast, quantitative reverse transcription – polymerase chain reaction (q RT-PCR or real-time RT-PCR) allows even weakly expressed genes to be accurately quantified .
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